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KMID : 0043319970200010013
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Archives of Pharmacal Research
1997 Volume.20 No. 1 p.13 ~ p.16
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Cytotoxicity and L-Amino Acid Oxidase Activity of Animal Venoms
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Ahn Mi-Young
Lee Byung-Mu
Kim Yeong-Shik
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Abstract
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The cytotoxicity of animal venoms (snakes, insects and marine animals) was measured against SNU-1 (stomach cancer cells) by dye uptake assay (MTT method). And also L-amino acid oxidase (AAO) activity of the venoms was compared. Among them, the venom from Ophiophagus hannah (king cobra) showed a strong AAO activity as well as a high potent cytotoxicity. Cytotoxic protein having a AAO was then partially purified by HPLC-GPC and two fractions (Fr. I and Fr. II) were collected. The values of Fr. I and Fr. II were 0.19 and 1.36 , respectively. The results suggested that the cytotoxicity of king cobra venom may be due to its AAO activity.
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KEYWORD
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Venoms, Cytotoxic protein, L-Amino acid oxidase
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